We've been hard at work to bring new functionality to your laboratory. Genotyper 3.1 resolves some bugs, improves user experience across the board and includes other general improvements.

As always, let us know what you think.

A passive reference dye (PRD) is used to normalize pipetting variance in your liquid handling equipment. Using a dye that always returns a fluorescence value, we can offset any pipetting variance by normalizing our main fluorescent signals (often, FAM & VIC).

Before 3.1, we visualized shortfall samples (i.e. a passive reference dye signal below a given threshold). With our newest release, we're giving you more granular control of the data.

Our new passive reference dye view allows you to analyze pipetting artefacts right on the plateview. Click on the PRD icon on top of the plate view. Both the plate- and plot-view will change datapoint colors. Switch back via the icon.

All PRD values in the plate are ranked from low to high and visualized with a light to dark color. Hover over each well to see the exact fluorescence values (only in the plate view, not in the plot view).
If datapoints on the plates are detected as being a shortfall, this is when the PRD-value < instrument threshold, a label will be visualized on the PRD-icon.

Failing and not exporting on a PCR plate level is now possible for all users (even non-Kraken ones). With this new feature, you can choose to fail one or more PCR plates or exclude the results from the export file completely.

Use the dropdown in the plate table for 1 plate:

Next to that, you can also do it at the level of all plates, simply use the dropdown in the upper right corner of the screen for one or multiple selected plates:

These options are now available:

We introduced many new settings to ensure the Genotyper export files (Universal and Grid export) fit into as many different laboratory workflows as possible.

The new settings available in 3.1 include:

A third option is introduced to sort your assays in the opened analysis view: sort by file order.

In the past, reviewing historically made decisions in Genotyper could be confusing. When opening an authorized analysis, you would see the 'today' history. Obviously, that removed the important historic context in which the AI and users took decisions.

Going forward, we're storing the history grids upon authorization, and visualize them at the point-in-time of authorization. Giving you the possibility to see data exactly the way your team/ AI saw it when analyzing the data.

This is a first important step in providing more, granular Metrics data for assay quality, more news on that soon.
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When a group of datapoints is selected with the lasso-tool, the manual override call will only affect the regular datapoints and the positive controls. NTCs will not be re-scored.

The result of an NTC can be edited by only selecting the NTC without any other datapoints.

This way, when a group of samples is rescored or unscored, uncalled NTCs (i.e. NTCs that passed) that lie within lassoed group will remain passed.

For input files with data of multiple reads, the number of reads analyzed by Genotyper is increased from 5 to 7. If more than 5 reads are available in your input file, you’ll notice the slider will now show up to 7 reads.

Customers using the Biomark (Fluidigm) instrument can now import the default multi-read file.

We are now only ~33 reads removed from giving you real-time PCR data.

The ‘add new file(s)’ functionality is improved to support more types of data.

Read more about partial importing in this knowledge base article.

Same as in the filters in the Analysis overview page, the filters in the Assay module are now remembered.
Set a filter, click around in Genotyper and go back to the assay module. You’ll see your filters are still in place.

Breed Bio's generic .gtd file parser has been updated to comply with more fluorescence reading devices. We now support:

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